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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 269

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Parathion	0.09	3.33	0.04	Ethanol	Pig	Shoulder	7.5	1	168	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
168	90.09	7.1	0.18036	60.12	0.00021	0.07	0.00156	0.52	0.0878	29.28	5.7	29.28

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					4

NOTES

The application site was occluded with a circular Hilltop chamber. 8-10 week old female weanling Yorkshire pigs were used. % Absorption was calculated from adding together the amounts exreted in the faeces and the urine. They did an iv dose for comparison and found that they recovered 100% in the faeces and urine so the data was not corrected for incomplete recovery. They also studied blood profiles but found that the radioactivity in the blood and plasma was too low to quantify throughout the entire topical experimental period. They also worked out maximal excretion rate & time of maximal excretion rate (see paper for data)

REFERENCE

Effects Of Anatomical Site And Occlusion On The Percutaneous Absorption And Residue Pattern Of 2,6-[ring-14C]Parathion In Vivo In Pigs (1993) Toxicol. Appl. Pharmacol., 122 :131-138