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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 285

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Dibutylphthalate	0.1	1050	10.5	None (Neat)	Rat	Back	10	2	72	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
72	94.5	1.1	13.97	13.3			7.88	7.5	77.28	73.6	8.2	73.6

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
	111				8	0.000106

NOTES

Sprague~Dawley Rats were used. The application site was protected with a perforated circular plastic cup. % Absorption was calculated by adding together the amounts found in the urine + faeces + carcass + cage wash. Results were given as +/- SEM. I have changed these to SD for the database

REFERENCE

In Vivo And In Vitro Percutaneous Absorption Of [14C]Di-N-Butylphthalate In Rat (2001) Drug Met. Dispos., 29 :843-853