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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 298

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
N,N-Diethyl-m-toluamide	0.05	1.092	0.013	Acetone	Rat	Mid-dorsal	4.2	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
336	83.8	9.23	0.0233	42.7			0.0001092	0.2	0.0224	41	10.51	41

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					4

NOTES

Sprague-Dawley Rats were used. The acetone vehicle was evaporated from the skin using an electric fan. The dose site was protected from surface rub-off by a foam rubber non-occlusive patch. % Absorption has been calculated from the amount found in the Urine + faeces + tissue.

REFERENCE

An Automated In Vitro Dermal Absorption Procedure: III. In Vivo and In Vitro Comparison With The Insect Repellent N,N-Diethyl-m-Tolumamide In Mouse, Rat, Guinea Pig, Pig, Human And Tissue Cultured Skin (1993) Toxicol. Vitro, 7 :167-176