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IN VIVO STUDIES FURTHER DETAILS


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In Vivo Study No: 299

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
N,N-Diethyl-m-toluamide0.051.0920.0125AcetoneGuinea pigMid-dorsal4.2224Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
336107.82.870.040877.8  5E-050.10.0158305.9630
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     4  

NOTES

The acetone vehicle was evaporated from the skin using an electric fan. Female Hairless Guinea Pigs were used. The dose site was protected from surface rub-off by a foam rubber non-occlusive patch. % Absorption has been calculated from the amount found in the Urine + faeces + tissue.

REFERENCE

An Automated In Vitro Dermal Absorption Procedure: III. In Vivo and In Vitro Comparison With The Insect Repellent N,N-Diethyl-m-Tolumamide In Mouse, Rat, Guinea Pig, Pig, Human And Tissue Cultured Skin (1993) Toxicol. Vitro, 7 :167-176