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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 304

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
DDT	0.05	0.5124	0.0061	Acetone	Rat	Mid-dorsal	4.2	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
336	104.3	3.92	0.0065	25.4					0.0185	72.1	5.85	72.1

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					4

NOTES

Male Sprague Dawley Rats were used. The acetone vehicle was evaporated from the skin using an electric fan. The dose site was protected from surface rub-off by a foam rubber non-occlusive patch. % Absorption has been calculated from the amount found in the Urine + faeces + tissue. There was some discrepancy in the values found in the skin so I have not include that in the database.

REFERENCE

In Vitro Dermal Absorption Of Pesticides VI. In Vivo And In Vitro Comparison Of The Organochlorine Insecticide DDT In Rat, Guinea Pig, Pig, Human and Tissue Cultured Skin (1994) Toxicol. Vitro, 8 :1225-1232