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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 305

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
DDT	0.05	0.7644	0.0091	Acetone	Guinea pig	Mid-dorsal	4.2	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
336	100	3.67	0.01445	37.8					0.0238	62.3	4.12	62.3

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					4

NOTES

Hairless Female Guinea Pigs were used. The acetone vehicle was evaporated from the skin using an electric fan. The dose site was protected from surface rub-off by a foam rubber non-occlusive patch. % Absorption has been calculated from the amount found in the Urine + faeces + tissue.

REFERENCE

In Vitro Dermal Absorption Of Pesticides VI. In Vivo And In Vitro Comparison Of The Organochlorine Insecticide DDT In Rat, Guinea Pig, Pig, Human and Tissue Cultured Skin (1994) Toxicol. Vitro, 8 :1225-1232