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IN VIVO STUDIES FURTHER DETAILS

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In Vivo Study No: 308

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
2,4 Dimethylamine	0.1	9.723	0.2315	Commercial Formulation Wilbur-Ellis	Rat	Mid-dorsal	4.2	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
168	106.2	2.95	0.881	90.6			0.058	6	0.1517	15.6	5.63	15.6

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					4

NOTES

Sprague-Dawley Rats were used. The % absorption was determined from the amounts found in the urine + faeces + tissue. A non-occclusive foam rubber skin patch was used to protect the site of application.

REFERENCE

In Vitro Dermal Absorption Of Two Commercial Formulations Of 2,4-Dichlorophenoxyacetic Acid Dimethylamine (2,4-D Amine) in Rat, Guinea Pig and Human Skin (1997) Toxicol. Vitro, 11 :251-262