EDETOX logo

IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 309

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Arsenic	See Notes		0.0006	Soil	Monkey	Abdomen	12	2	24	Radiolabelled (73As)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
168				76.6					0.00023	3.2	1.9	3.2

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

168

76.6

0.00023

3.2

1.9

3.2

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					4

NOTES

The soil was kept on the skin using a non-occlusive gortex and aluminium eye patches with large holes drilled into them. The soil sample came form Yolo County, California (26% sand, 26% clay, 48% silt 0.9% organic) 4.8g of soil was used. The % absorption was calculated from the amount present in the urine. This was corrected for incomplete excretion by comparing with the amount excreted after an intravenous dose.

REFERENCE

In Vivo And In Vitro Percutaneous Absorption And Skin Decontamination Of Arsenic From Water And Soil (1993) Fundam. Appl. Toxicol., 20 :336-340