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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 317

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Caffeine	0.2	0.5	0.004	Ethanol	Pig	Upper Back	25	2	48	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
	90	6	0.066	66			0.0021	2.1	0.02	20		20

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					3

NOTES

Female Weanling Yorkshire type Pigs were used. A non-occlusive patch was used to protect the application site.% absorption was calculated by adding together the amounts found in the faeces + urine. Measurements were taken until excreted amounts reached background levels.

REFERENCE

Evaluation Of Animal Models For Predicting Skin Penetration in Man (1984) Fundam. Appl. Toxicol., 4 :224-230