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In Vivo Study No: 32

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
Benzoic Acid0.010.5080.004EthanolMouseAnterior End1.27224Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
 8930.0017534.4  3E-050.50.0027554.2 54.2
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     3  

NOTES

Athymic, Hairless Mice were used. Radioactivity was measured until the amount present in the urine was equal to background levels. The % Absorption was derived by adding the % of the topically applied radioactive dose in urine, faeces & carcass - application site. Radioactive dose 0.05microCi.

REFERENCE

Percutaneous Penetration In The Hairless Dog, Weanling Pig And Grafted Athymic Nude Mouse (1984) Br. J. Dermatol., 111 :123-135