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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 329

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Diethyl Malonate	Not Given		0.1	None (Neat)	Dog	Back	25	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
									0.1	4	2	4

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

0.1

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					3

NOTES

Hairless Dogs were used. A non-occlusive patch was used to protect the application site.% absorption was calculated by adding together the amount excreted in the urine and the faeces and correcting for incomplete excretion by comparison with a parentally administered dose

REFERENCE

Evaluation Of Animal Models For Predicting Skin Penetration in Man (1984) Fundam. Appl. Toxicol., 4 :224-230