EDETOX logo

IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 341

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Malathion	0.2	1	0.04	Ethanol	Pig	Abdomen	5	2	144	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
144	92.2	14.9							0.0038	1.9		1.9

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

144

92.2

14.9

0.0038

1.9

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					3

NOTES

Female Weanling Yorkshire Pigs were used. The abdominal area was lightly shaved prior to application of the dose compound. The application site was enclosed with a foam rubber border, this was covered with a nylon screen and a non-occlusive gauze pad. Total absorption was calculated by adding together the amounts found in the urine + faeces + carcass.

REFERENCE

Percutaneous Absorption And Excretion Of Xenobiotics After Topical And Intravenous Administration To Pigs (1989) Fundam. Appl. Toxicol., 13 :714-722