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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 349

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Caffeine	See Notes		0.00325	Petrolatum	Rat	Back	2	2	120	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
120

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

120

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					7	0.00021

NOTES

Dononr concentration was 130ng/mg. 50mg were added in total. Female-Osborne Mendel Rats 3-6 months old were used. A wire mesh cover was put over the exposure site. The rats were anaethitized throughout the experiment. Urine was collected by annulating the bladder with PE-50 polyethylene tubing so that urine could be collected as it was produced. Bllod samples were collected from the tail vein in heparinized capilary tubes.

REFERENCE

Methods For In Vitro Percutaneous Absorption Studies I. Comparison With In Vivo Studies (1982) Toxicol. Appl. Pharmacol., 62 :474-480