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In Vivo Study No: 353

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
Acetylsalicylic AcidInfinite 0.00625PetrolatumRatBack22120Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
120        0.003124.89.824.8
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     5  

NOTES

The donor concentration was 250ng/mg. 50mg were added in total. Female-Osborne Mendel Rats 3-6 months old were used. A wire mesh cover was put over the exposure site. The legs of each rat were prevented from scratching the application site by wrapping rubber tubing around the body. The rats were placed in separate metabolism cages for collection of 24hr urine samples. The values were corrected for incomplete urinary excretion, using percentages for recovery in urine after iv administration.

REFERENCE

Methods For In Vitro Percutaneous Absorption Studies I. Comparison With In Vivo Studies (1982) Toxicol. Appl. Pharmacol., 62 :474-480