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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 362

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Cypermethrin	0.003	359	1.077	None (Neat)	Rat	dorso-lumbar region	1	2	8	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
8									0.0109	1	0.69	1

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

0.0109

0.69

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					3

NOTES

4-5 week old Alpk/AP strain Wistar derived rats were used. The fur from the dorso-lumbar region was removed using clippers 20-20h before dosing.A plastic guard was glued around the application site using cyanoacrylate ester glue. Back scratching was prevented by tying rubber tubing around the body. % absorption was calculated from the amounts found in blood samples, urine, faeces and the carcass of the rats.

REFERENCE

Comparison Of The In Vivo And In Vitro Percutaneous Absorption Of A Lipophilic Molecule (Cypermethrin, a Pyrethroid Insecticide) (1987) J. Investig. Dermatol., 89 :142-146