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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 38

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
N,N-Diethyl-m-toluamide	0.01	0.508	0.004	Ethanol	Human skin grafted mouse	Breast/Abdomen/Face	1.27	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
	81	5	0.00279	55			2E-05	0.3	0.00131	25.8		25.8

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

0.00279

2E-05

0.3

0.00131

25.8

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					3

NOTES

Athymic Nude Mice were used. For successful grafting a portion of the dermis was removed with a dermatome. The final thickness of the grafted skin was about 0.7mm. The graft was positioned laterally towards the anterior end of the mouse and 6 weeks were allowed for the graft to heal before the start of the experiment. A non-occlusive patch was used to protect the application site.% absorption was calculated by adding together the amounts found in the faeces + urine and carcass - application site. Measurements were taken until excreted amounts reached background levels.

REFERENCE

Percutaneous Penetration In The Hairless Dog, Weanling Pig And Grafted Athymic Nude Mouse (1984) Br. J. Dermatol., 111 :123-135