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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 381

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Diethyl Malonate	0.02	0.5	0.004	Acetone	Human	Forearm	2.5	1	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
168									0.00543	54.3	17.9	54.3

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

168

0.00543

54.3

17.9

54.3

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					6

NOTES

The ste was occluded with a polypropylene Hill Top chamber. The chamber was removed at 24 h to allow washing of the site and then reapplied for the following 6 days. The results were given +/- SEM. I have converted these to SD for the purpose of this database. % Absorption was determined by measuring the radioactivity recovered in the urine samples, and then correcting the amount of radioactivity for complete excretion with a correction factor obtained from a parentally administered dose, administered to Rhesus monkeys

REFERENCE

In Vivo Percutaneous Absorption Of Fragrance Ingredients In Rhesus Monkeys And Humans (1990) Food Chem.Toxicol., 28 :369-373