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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 401

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Benzyl Alcohol	See Notes		0.004	Acetone	Monkey	Abdomen	1	1	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
120									0.0032	79.9	14.8	79.9

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

120

0.0032

79.9

14.8

79.9

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					4

NOTES

The application site was occluded with a glass chamber covered with parafilm. 4 female rhesus monkeys were used in this study, the monkeys were restrained in metabolic chairs for the duration of the study, except when the washing procedure was taking place. Between 10 and 20 mcrl were added in each study. The results were given +/- SEM. I have converted these to SD for the purpose of this database. % Absorption was determined by measuring the radioactivity recovered in the urine samples, and then correcting the amount of radioactivity for complete excretion with a correction factor obtained from a parentally administered dose.

REFERENCE

In Vivo Percutaneous Absorption Of Fragrance Ingredients In Rhesus Monkeys And Humans (1990) Food Chem.Toxicol., 28 :369-373