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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 477

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Chlorpyrifos	0.1	5.413	0.1933	Acetone	Rat	Mid-dorsal	2.8	2	72	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
72	79.3	2.8							0.1147	21.2	1	21.2

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

79.3

2.8

0.1147

21.2

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					3

NOTES

Young female Fischer 344 Rats were used. The fur on the backs of the animals was removed with an electric animal hair clipper 24h prior to treatment. The treated area was protected by gluing a 5ml perforated plastic beaker with cyanoacrylate glue. The rats were housed in metabolism cages equipped with devices for urine and faeces collection. The results were given as fractional penetration of the compound. This was found by divinding the % applied radioactivity recovered in the urine and faeces by the percentage radioactivity recovered. I have multiplied this back up to give the actual % recovered in the urine and faeces. The results were also gived +/- SEM. I have converted this to SD for the purpose of this database.

REFERENCE

Comparison Of The Penetration Of 14 Pesticides Through The Skin Of Young And Adult Rats (1987) J. Toxicol. Environ. Health, 21 :353-366