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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 521

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Octyl benzoate	0.1	0.029	0.000725	Ethanol	Rat	Not Given	4	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
24									0.00113	39		39

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

0.00113

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)

NOTES

7 week old Alderly Park Witar derived rats were used. The % Absoprtion was calculated from the amount excreted in the urine. They did also do an iv dose but there is no evidence to suggest that the % absorption given for absorption through skin had been corrected for incomplete excretion by comparison to this dose. They did not mention how many rats were used in this study or the application site.

REFERENCE

A Model For Quantifying Absorption Through Abnormal Skin (1986) J. Investig. Dermatol., 86 :208-212