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IN VIVO STUDIES FURTHER DETAILS


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In Vivo Study No: 525

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
Coumarin0.20.810.01870% aq. EthanolRatBack916Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
4883.3 0.022714  0.00583.580.10564.8 64.8
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     2  

NOTES

Male Lister Hooded (pigmented) rats were used. The solvent was allowed to evaporate and then th application site was occluded with aluminium foil. The skin was wiped with a cotton wool swab moistened with 70% etjanol after 6h. Then the site was re-occluded with clean aluminium foil. % Absorption was calculated from the amount found in the: urine + faeces + expired air + tissues

REFERENCE

The In Vivo Dermal Absorption And Metabolism Of [4-14C]coumarin By Rats And By Human Volunteers Under Simulated Conditions Of Use In Fragrances (2001) Food Chem.Toxicol., 39 :153-162