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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 528

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Coumarin	1	1.9	0.019	70% aq. Ethanol	Human	Back	100	2	6	Radiolabelled (14C).

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
120	66.3	1.8	0.1197	6.3	0.0182	0.96			1.1343	59.7	4.5	59.7

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					3

NOTES

3 healthy male volunteers were used in this study. The solvent was allowed to evaporate off in 30mins and then a gauze dressing was applied. 6h after application the residual coumarin was wiped off with 705 ethanol. The % absorption was calculated from the % recovered in the: urine + faeces

REFERENCE

The In Vivo Dermal Absorption And Metabolism Of [4-14C]coumarin By Rats And By Human Volunteers Under Simulated Conditions Of Use In Fragrances (2001) Food Chem.Toxicol., 39 :153-162