EDETOX logo

IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 529

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
AHTN	0.2	4.5	0.1	70% aq. Ethanol	Rat	Back	9	1	0.5	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
0.5	92.7		0.7686	85.4			0.0607	6.74	0.005	0.55		0.55

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					2

NOTES

Lister-Hooded (pigmented) rats were used. After dosing the application area was occluded with aluminium foil. % absorption was calculated from the amounts recovered in the tissues. Excreta were not measured due to the short duration of the experiment.

REFERENCE

The Systemic Exposure To The Polycyclic Musks, AHTN and HHCB, Under Conditions Of Use As Fragrance Ingredients: Evidence Of Lack Of Complete Absorption From A Skin Reservoir (1999) Toxicol. Lett., 111 :133-142