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In Vivo Study No: 535

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
AHTN0.24.50.170% aq. EthanolRatBack916Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
4891.5 0.643571.5  0.04014.450.139515.5 15.5
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     2  

NOTES

Lister-Hooded (pigmented) rats were used. After dosing the application area was occluded with aluminium foil. % absorption was calculated from the amounts recovered in the: urine + faeces + cage wash + tissues. After 6h the occlusion was removed and retained. The residual dose material was removed from the skin with cotton wool swabs moistened with 70% aq. Ethanol. The treated area was then re-occluded with clean aluminium foil.

REFERENCE

The Systemic Exposure To The Polycyclic Musks, AHTN and HHCB, Under Conditions Of Use As Fragrance Ingredients: Evidence Of Lack Of Complete Absorption From A Skin Reservoir (1999) Toxicol. Lett., 111 :133-142