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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 550

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
DEHP	0.1	9.52	0.119	Acetone	Guinea pig	Dorsal	8	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
24	85.2	2.9	0.6397	67.2			0.1057	11.1	0.1219	12.8	3.6	12.8

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					4

NOTES

Female Hartley Hairless Guinea Pigs were used. 2 separate dosing sites each 4cm2 were used. 50 mcrl of the dose was applied to each site. After the acetone had evaporated a 6x10cm foam pad with an approximately 3x6cm opening covered with gauze was placed over the dosing sites and was fixed in place with a Vertrap bandage. % absorption was calculated by adding together the amounts recovered in the urine = faeces + carcass + dosed skin. The amount recovered in the urine was not given separately, but the amount recovered in the urine + faeces was: 0.74%

REFERENCE

Skin Reservoir Formation And Bioavailability Of Dermally Administered Chemicals In Hairless Guinea Pigs (1996) Food Chem.Toxicol., 34 :267-276