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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 553

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
DEHP	0.1	8.56	0.107	Acetone	Guinea pig	Dorsal	8	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
48	71	9.2	0.4417	51.6			0.0873	10.2	0.1592	18.6	4.6	18.6

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					4

NOTES

Female Hartley Hairless Guinea Pigs were used. 2 separate dosing sites each 4cm2 were used. 50 mcrl of the dose was applied to each site. After the acetone had evaporated a 6x10cm foam pad with an approximately 3x6cm opening covered with gauze was placed over the dosing sites and was fixed in place with a Vertrap bandage. % absorption was calculated by adding together the amounts recovered in the urine = faeces + carcass + dosed skin. The amount recovered in the urine was not given separately, but the amount recovered in the urine + faeces was: 6.1% The skin was washed 3x with 1% soap solution and 3x with water after 24hours to remove any of the dose remaining on the surface

REFERENCE

Skin Reservoir Formation And Bioavailability Of Dermally Administered Chemicals In Hairless Guinea Pigs (1996) Food Chem.Toxicol., 34 :267-276