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IN VIVO STUDIES FURTHER DETAILS


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In Vivo Study No: 559

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
Benzo[a]pyrene0.11.720.0215AcetoneGuinea pigDorsal8224Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
2484.913.60.100658.5  0.01639.50.045426.45.526.4
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     4  

NOTES

Female Hartley Hairless Guinea Pigs were used. 2 separate dosing sites each 4cm2 were used. 50 mcrl of the dose was applied to each site. After the acetone had evaporated a 6x10cm foam pad with an approximately 3x6cm opening covered with gauze was placed over the dosing sites and was fixed in place with a Vertrap bandage. % absorption was calculated by adding together the amounts recovered in the urine = faeces + carcass + dosed skin. The amount recovered in the urine was not given separately, but the amount recovered in the urine + faeces was: 4.6%

REFERENCE

Skin Reservoir Formation And Bioavailability Of Dermally Administered Chemicals In Hairless Guinea Pigs (1996) Food Chem.Toxicol., 34 :267-276