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IN VIVO STUDIES FURTHER DETAILS


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In Vivo Study No: 563

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
Benzo[a]pyrene0.050.7640.0091AcetoneGuinea pigmid-dorsal4.2224Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
336100.62.26    4E-050.10.025967.89.3367.8
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     4  

NOTES

Hairless Female Guinea Pigs were used. The acetone vehicle was evaporated from the skin using an electric fan. The dose site was protected from surface rub-off by a foam rubber non-occlusive patch. After 24h the application site was washed with radiac soap solution. % Absorption has been calculated from the amount found in the urine + faeces + tissue. Extra experimental detail was obtained from ref no 90.

REFERENCE

In Vivo And In Vitro Dermal Absorption Of Benzo[a]pyrene In Rat, Guinea Pig, Human And Tissue Cultured Skin (1995) J. Dermatol. Sci., 9 :48-58