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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 564

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Benzo[a]pyrene	0.05	0.512	0.0061	Acetone	Rat	mid-dorsal	4.2	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
336	102.2	4.81					5E-05	0.2	0.0174	67.8	9.33	67.8

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

336

102.2

4.81

5E-05

0.2

0.0174

67.8

9.33

67.8

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					4

NOTES

5 wekk-old male Sprague Dawley Rats erre used. The acetone vehicle was evaporated from the skin using an electric fan. The dose site was protected from surface rub-off by a foam rubber non-occlusive patch. After 24h the application site was washed with radiac soap solution. % Absorption has been calculated from the amount found in the urine + faeces + tissue. Extra experimental detail was obtained from ref no 90.

REFERENCE

In Vivo And In Vitro Dermal Absorption Of Benzo[a]pyrene In Rat, Guinea Pig, Human And Tissue Cultured Skin (1995) J. Dermatol. Sci., 9 :48-58