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In Vivo Study No: 567

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
DEHP0.054.240.053AcetoneGuinea pigDorsal4224Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
16895.215.10.065330.80.02411.3  0.112653.113.953.1
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     5  

NOTES

Female Hairless Guinea Pigs were used. The application site was covered with a non-occlusive pad to prevent the animals from ingesting the compound. % absorption was calculated from the addition of amounts excreted in the urine and faeces. This data has been corrected for incomplete excretion by comparison with the amounts excreted after an im dose.

REFERENCE

Percutaneous Absorption And Metabolism Of Pyrene, Benzo[a]pyrene, and Di(2-ethylhexyl) Phthalate: Comparison Of In Vitro And In Vivo Results In The Hairless Guinea Pig (1992) Toxicol. Appl. Pharmacol., 115 :216-223