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In Vivo Study No: 593

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
Isofenphos0.062.640.0132AcetoneHumanVentral Forearm12224Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
168  0.00150.75 0.003  0.00573.633.583.63
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     4  

NOTES

The dose was allowed to dry on the skin (5 min) and then the volunteers were allowed to cover the application site with clothing. The dose site was washed at 24h. The skin was stripped after 7-days to calculate the amount retained in the Stratum Corneum. An IV dose was given to Rhesus Monkeys and the amount excreted in the urine after 7 days was calculated this was then used to correct for incomplete excretion in the urine by man to give the % absorption. The % recovery was extremely low.

REFERENCE

In Vivo And In Vitro Percutaneous Absorption And Skin Evaporation Of Isofenphos In Man (1992) Fundam. Appl. Toxicol., 19 :521-526