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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 643

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Testosterone	Not Given		0.004	Methanol	Monkey	Ventral forearm	1	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
96									0.00084	21.1		21.1

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

0.00084

21.1

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					3

NOTES

Newborn mokeys were used 2 hours after delivery. Both male and female monkeys were used. The arm was lightly shaved prior to application of the compound. They were kept in metabolism chairs with their arms secured to prevent them from wiping off the applied dose. After 24 hours the site was washed with soap and water but urine collection continued. % absorption was calculated from the amount excreted in the urine, corrected for incomplete excretion by comparison with the amount excreted after an intravenous dose. The amount excreted from an iv dose after 5 days was 71.1%

REFERENCE

Percutaneous Absorption Of Testosterone In The Newborn Rhesus Monkey: Comparison To The Adult (1977) Pediat. Res., 11 :737-739