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IN VIVO STUDIES FURTHER DETAILS


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In Vivo Study No: 654

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
Benzoic Acid0.020.50.004AcetoneHumanVentral forearm2.5224Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
16845.67.4      0.0036236.212.236.2
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     7  

NOTES

Young Caucasian Adults were used. (22-40 years) The application site was covered with a protective but non-occlusive device. A Hilltop chamber was modified by boring 6 holes thtough the device and the cotton pads were removed and a goretex membrane was inserted. After 24 hours the site was washed and the protective patch removed. Another patch was added and urine collection continued for 7 days. Absorption values were obtained from the amount recovered in the urine corrected for incomplete excretion by comparison with the amount excreted after an iv dose. Results were given as SEM. I have converted these to SE for the purpose of this database.

REFERENCE

The Effect Of Aging On Percutaneous Absorption In Man (1989) J. Pharm. Biopharm., 17 :617-630