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In Vivo Study No: 694

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
2-Nitro-4-Phenylenediamine0.14.810.048150% aq. Hair colourant baseRatBack1020.083Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
48100.34.70.36876.6  0.09720.170.0173.530.623.53
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     3  

NOTES

Female Colworth Wistar Rats were used. After the application to the skin, the area was lightly massaged with a rounded glass rod for 1 min to produce a lather. The treated area was left for a further 4 mins before being rinsed with an excess of distilled water. The skin was blotted dry with paper tissues and then protected with a non-occlusive patch. % absorption includes the amounts recovered in the excreta and that recovered from the carcass excluding the application site.

REFERENCE

Percutaneous Absorption Of 2-nitro-p-phenylenediamine (1983) Int. J. Cosm. Sci., 5 :215-226