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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 696

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
2-Nitro-4-Phenylenediamine	0.1	4.81	0.0481	50% aq. Hair colourant base	Rat	Back	10	1	0.083	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
48	98.5	2.2	0.327	68			0.105	21.83	0.042	8.73	2.91	8.73

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					3

NOTES

Female Colworth Wistar Rats were used. After the application to the skin, the area was lightly massaged with a rounded glass rod for 1 min to produce a lather. The treated area was left for a further 4 mins before being rinsed with an excess of distilled water. The skin was blotted dry with paper tissues and then protected with an occlusive patch. % absorption includes the amounts recovered in the excreta and that recovered from the carcass excluding the application site.

REFERENCE

Percutaneous Absorption Of 2-nitro-p-phenylenediamine (1983) Int. J. Cosm. Sci., 5 :215-226