EDETOX logo

IN VIVO STUDIES FURTHER DETAILS


BACK TO THE MAIN MENU

back to previous page forward to next page

University of Newcastle homepage

In Vivo Study No: 707

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
Acetylsalicylic Acid0.029.0080.18016ethyleneglycol:triton X-100 (90:10)RatBack120.5Radilabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
96    0.000930.5158E-050.0420.001460.8090.1670.809
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     5  

NOTES

Female hairless Sprague-Dawley rats were used. The application area was delimited by an open circular cell fixed by silicone glue to prevent spreading. % absorption was calculated by adding together the amounts found in the urine, faeces, epidermis + dermis of treated area and anumal body. The amount recovered in the stratum corneum was treated as unabsorbed.

REFERENCE

The Measurement Of The Stratum Corneum Reservoir. A Predictive Method For In Vivo Percutaneous Absorption Studies (1985) J. Investig. Dermatol., 84 :66-68