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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 713

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Nicotinic Acid	0.02	6.1555	0.12311	ethyleneglycol:triton X-100 (90:10)	Rat	Back	1	2	4	Radilabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
96							0.0006	0.487	0.0152	12.345	2.55	12.345

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

0.0006

0.487

0.0152

12.345

2.55

12.345

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					5

NOTES

Female hairless Sprague-Dawley rats were used. The application area was delimited by an open circular cell fixed by silicone glue to prevent spreading. % absorption was calculated by adding together the amounts found in the urine, faeces, epidermis + dermis of treated area and anumal body. The amount recovered in the stratum corneum was treated as unabsorbed.

REFERENCE

The Measurement Of The Stratum Corneum Reservoir. A Predictive Method For In Vivo Percutaneous Absorption Studies (1985) J. Investig. Dermatol., 84 :66-68