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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 719

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Trichloromethane	0.05	1	0.0161	Distilled water	Human	ventral forearm	3.1	2	8	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
72	48.4	4.33	0.00013	0.26	1E-05	0.01			0.0041	8.2	1.63	8.2

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					3

NOTES

A stainless steel skin-depot was affixed to the forearm using medical bandage tapes prior to the treatment. The top portion of the skin-depot contained 100-150mg of a solid sorbent (activated charcoal 20/40 mesh) to capture and recover the postion of the test dose which was lost by evaporation from the application site. After 8 h the skin depot was removed and the skin was cleansed with a 1% soap solution. % Absorption was calculated from the amounts recovered in the urine, exhaled air and skin strippings. Most of the recovered radioactivity was from the vapor trap - 48.4%

REFERENCE

In Vitro And In Vivo Percutaneous Absorption Of 14C-Chloroform In Humans (1995) Hum. Exp. Toxicol., 14 :260-265