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IN VIVO STUDIES FURTHER DETAILS


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In Vivo Study No: 720

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
Trichloromethane0.0550.0806EthanolHumanventral forearm3.128Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
7222.144.555E-050.0200  0.00421.680.361.68
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     4  

NOTES

A stainless steel skin-depot was affixed to the forearm using medical bandage tapes prior to the treatment. The top portion of the skin-depot contained 100-150mg of a solid sorbent (activated charcoal 20/40 mesh) to capture and recover the postion of the test dose which was lost by evaporation from the application site. After 8 h the skin depot was removed and the skin was cleansed with a 1% soap solution. % Absorption was calculated from the amounts recovered in the urine and exhaled air. Most of the recovered radioactivity was from the vapor trap - 20.44%

REFERENCE

In Vitro And In Vivo Percutaneous Absorption Of 14C-Chloroform In Humans (1995) Hum. Exp. Toxicol., 14 :260-265