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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 748

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
2-phenylphenol	0.1	12	0.12	60% aq. Ethanol	Rat	dorsal	10	1	4	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
24			0.531	44.25					0.429	35.75	3.08	35.75

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

0.531

44.25

0.429

35.75

3.08

35.75

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
							0

NOTES

Young male albino Wistar rats were used. 5 min after application the dose site was covered with an elastic adhesive bandage, small perforations were made in the bandage so the conditions were semi-occlusive. Ingestion of the test substance was prevented by a collar around the rats neck. After the exposure period of 4h the cover was removed and retained for analysis, the application site was cleaned 5 times using cotton swabs. After cleaning a fresh cover tape was applied to the O-ring and urine and faeces collection continued. % absorbed was calculated from the amounts recovered in the urine and the faeces.

REFERENCE

Comparative In Vitro-In Vivo Percutaneous Penetration Of The Fungicide Ortho-Phenylphenol (2002) Regul. Toxicol. Pharmacol., 35 :198-208