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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 753

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
2-phenylphenol	0.3	40	0.12	60% aq. Ethanol	Human	Volar forearm	100	2	4	Capillary GC + Mass-selective detection

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
48			5.31	44.25					3.2	26.67	1.83	26.67

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

5.31

44.25

3.2

26.67

1.83

26.67

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
11					3	0.0158	0.94

NOTES

3 male volunteers (23-24 years old) were used in this study. After 4 h the excess of the applied substance was washed off by wiping 10 times with dentists cotton wool balls. During the exposure the subjects were housed in a cabin with their forearm placed into an incubator, both cabin and incubator were controlled for temperature (25oC) and humidity (50 +/- 10%) The absorbed dose was calculated by dividing the dermal absorption value by the fraction of an administered iv dose that was excreted in the urine. Before analysis using GC-MS samples were treated with acid tin order to hydrolyse glucuronide and sulfate conjugates to form the parent compound.

REFERENCE

Comparative In Vitro-In Vivo Percutaneous Penetration Of The Fungicide Ortho-Phenylphenol (2002) Regul. Toxicol. Pharmacol., 35 :198-208