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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 76

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Sodium Salicylate	Not Given		0.03202	Aq. Ethanol	Rat	Back	1	2	0.5	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
96							0.00016	0.5	0.0008	2.5	0.35	2.5

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

0.00016

0.5

0.0008

2.5

0.35

2.5

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					6

NOTES

Sprague-Dawley hairless Rats were used. Amount added was given as 0.2micromol/cm2. % Absorption is gathered from urine + faeces + sacrificed body including the epidermis & dermis of the application site.

REFERENCE

An Original Predictive Method For In Vivo Percutaneous Absorption Studies (1987) J. Soc. Cosmet. Chem., 38 :397-417