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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 766

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Benzoic Acid	0.02	0.7631	0.01526	ethylene glycol:10%Triton X-100	Human	Arm	1	2	0.5	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
48									0.00011	0.74	0.05	0.74

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

0.00011

0.74

0.05

0.74

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					5

NOTES

Caucasian males were used. The application area was delimited by an open cell fixed with sillicon glue. After 30min the application site was washed twice with 95% aq. Ethanol followed by 2 rinsings with water and light drying with cotton wool. % Absorption was taken as the amount recovered in the urine.

REFERENCE

In Vivo Relationship Between Horny Layer Reservoir Effect And Percutaneous Absorption In Human And Rat (1984) J. Investig. Dermatol., 82 :353-356