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In Vivo Study No: 772

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
Benzoic Acid0.026.1050.1221ethylene glycol:10%Triton X-100HumanArm120.5Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
48        0.000870.710.210.71
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     7  

NOTES

Caucasian males were used. The application area was delimited by an open cell fixed with sillicon glue. After 30min the application site was washed twice with 95% aq. Ethanol followed by 2 rinsings with water and light drying with cotton wool. % Absorption was taken as the amount recovered in the urine.

REFERENCE

In Vivo Relationship Between Horny Layer Reservoir Effect And Percutaneous Absorption In Human And Rat (1984) J. Investig. Dermatol., 82 :353-356