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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 794

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Sodium Lauryl Sulphate	0.5	7.28	0.364	Water	Rat	Back	10	2	0.25	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
24							0.036	0.1	0.0026	0.71	0.02	0.71

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

0.036

0.1

0.0026

0.71

0.02

0.71

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					6

NOTES

Female Colworth Wistar Rats weighing 100-120g were used. All animals were lightly anaesthetized during the experiments. After 15min contact the animal was inverted over a 6-inch diameter funnel and the excess of test solution was rinsed off with distilled water at 37oC. % Absorption was calculated from the levels of 14C recovered in the expired air, urine, faeces and the carcass after excision of the treated area of skin.

REFERENCE

The Percutaneous Absorption Of Some Anionic Surfactants (1975) J. Soc. Cosmet. Chem., 26 :47-63