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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 801

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Anthracene	0.071	0.16	0.0093	Hexane:Acetone (1:7)	Rat	Dorsal	1.23	2	72	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
72									0.00455	39.8	4.9	39.8

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

0.00455

39.8

4.9

39.8

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					4

NOTES

Female Sprague-Dawley Rats were used. A nylon cell was attached to the skin with cyanoacrylate and epoxy adhesives. The concentration was given as ABOUT 0.2mg/ml, however this figure does not fit in with the other figures given. So I have changed it to 0.16 as this was the only figure given as an approximate. % Absorption is calculated from the amount recovered in the urine and the faeces.

REFERENCE

Percutaneous Absorption Of Anthracene In The Rat: Comparison Of In Vivo And In Vitro Results (1986) Toxicol. Ind. Health, 2 :79-84