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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 807

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Salicylamide	Not Given		3	dimethylisosorbide + 2-propanol	Rat	Flank	5.08	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
24			3.66	24	0.46	3	0.27	1.8	10.85	71.2		71.2

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					5

NOTES

Female Sprague Dawley Rats were used. The application sites were not occluded but a self-adhesive foam pad, supported by a teflon ring was secured around each site. 2sites each of 2.54cm2 were used. The % absorbed was calculated as the amount applied minus the amount recovered on the surface and in the skin.

REFERENCE

In Vivo Skin Penetration of Salicylic Compounds in Hairless Rats (2002) Eur. J. Pharm. Sci., 17 :95-104