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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 809

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Cortisone				Petrolatum	Rat	Back	1.25	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
										19.9	1.3	19.9

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

19.9

1.3

19.9

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					4

NOTES

Rats were restrained by rubber tubing tied around the body behind the forelegs and in front of the rear legs. The back of each rat was shaved with electric clippers. A nylon ring was attached to the back by cyanoacrylate adhesive. A parenteral dose was administered separately so that results could be corrected for incomplete urinary excretion.

REFERENCE

Methods For In Vitro Percutaneous Absorption Studies V: Permeation Through Damaged Skin (1985) J. Pharm. Sci., 74 :1062-1066