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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 814

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Toluene	0.00449	866.4	4.86	None (Neat)	Mouse	Back	0.8	2	4	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
4	95	3.46	3.633	83.8					0.08967	2.1	1.73	2.1

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
	2940	2271			12

NOTES

Male Albino Hairless Mice were used (HRS/J) Stainless steel skin depots were glued to the backs of the mice using cyanoacrylate. Results were given +/- SEM. I have converted these to SD for this database. The % Absorbed was the sum of the mean percentages recovered in the exreta, carcass, skin application site and expired breath. They were aiming to add 5mcrl of solution but about 5-10% was retained by the application needle resulting in the average volume applied being slightly less than this. The concentration added was extrapolated back from the amount applied.

REFERENCE

In Vivo Percutaneous Absorption Studies of Volatile Organic Solvents In Hairless Mice II. Toluene, Ethylbenzine and Aniline (1990) J. Appl. Toxicol., 10 :217-225