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In Vivo Study No: 816

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
Aniline0.004581021.85.85None (Neat)MouseBack0.824Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
494.93.164.09881.8    0.22314.73.164.7
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
 135.6101.4  10  

NOTES

Male Albino Hairless Mice were used (HRS/J) Stainless steel skin depots were glued to the backs of the mice using cyanoacrylate. Results were given +/- SEM. I have converted these to SD for this database. The % Absorbed was the sum of the mean percentages recovered in the exreta, carcass, skin application site and expired breath. They were aiming to add 5mcrl of solution but about 5-10% was retained by the application needle resulting in the average volume applied being slightly less than this.The concentration added was extrapolated back from the amount applied.

REFERENCE

In Vivo Percutaneous Absorption Studies of Volatile Organic Solvents In Hairless Mice II. Toluene, Ethylbenzine and Aniline (1990) J. Appl. Toxicol., 10 :217-225